Takagi K, Yamashita Y, Inouye H, Ohseto M, Kuwabara H, Nishio O, Isomura S
Department of Microbiology, Ehime Prefectural Institute of Public Health.
Kansenshogaku Zasshi. 1991 May;65(5):552-8. doi: 10.11150/kansenshogakuzasshi1970.65.552.
We were prepared three monoclonal antibodies in which the monoclone 12D was type specific for Adenovirus 40 (Ad40), 1F was type specific for Ad41 and 15D was group specific for Ads. For identification of enteric adenoviruses (EAd) in stool specimens, enzyme-linked immunosorbent assay (ELISA) test using monoclonal antibodies was developed. Results of identification by the ELISA tests using monoclonal antibodies to EAd on 15 fecal samples in which Ad particles were found by electron microscopy showed complete coincidence to those of Sma 1 restriction endonuclease cleavage. From these results, the ELISA tests employing EAds type specific monoclonal antibodies proved to be specific and this was a rapid technique for laboratory diagnosis of EAd in fecal specimens of viral gastroenteritis. Fifty-eight fecal samples with Ad particles positive by EM were serotyped by the ELISA using monoclonal antibodies. Eleven fecal samples were identified as Ad40, 25 as Ad41, 1 as double infection with Ad40 and Ad41, and 4 as non-EAd. These results indicated that Ad41 was more dominant than Ad40 during April, 1986 to January, 1989 in Matsuyama city.
我们制备了三种单克隆抗体，其中单克隆抗体12D对腺病毒40（Ad40）具有型特异性，1F对Ad41具有型特异性，15D对腺病毒属具有群特异性。为了鉴定粪便标本中的肠道腺病毒（EAd），开发了使用单克隆抗体的酶联免疫吸附测定（ELISA）试验。在15份通过电子显微镜发现有腺病毒颗粒的粪便样本上，使用针对EAd的单克隆抗体进行ELISA试验的鉴定结果与Sma 1限制性内切酶切割的结果完全一致。根据这些结果，采用EAd型特异性单克隆抗体的ELISA试验被证明具有特异性，这是一种用于病毒性胃肠炎粪便标本中EAd实验室诊断的快速技术。通过使用单克隆抗体的ELISA对58份经电子显微镜检测腺病毒颗粒呈阳性的粪便样本进行血清分型。11份粪便样本被鉴定为Ad40，25份为Ad41，1份为Ad40和Ad41双重感染，4份为非EAd。这些结果表明，在1986年4月至1989年1月期间，松山市Ad41比Ad40更占优势。
