Weber C, Jakobsen T S, Mortensen S A, Paulsen G, Hølmer G
Medical Department B, State University Hospital (Rigshopitalet), Copenhagen, Denmark.
Mol Aspects Med. 1994;15 Suppl:s97-102. doi: 10.1016/0098-2997(94)90018-3.
A human study including 22 volunteers was conducted to investigate the antioxidative effect in blood of dietary coenzyme Q10 supplementation. The levels of alpha-tocopherol, ascorbic acid, lipid peroxidation (measured as TBARS) and the redox status of CoQ10 (reduced CoQ10/total CoQ10) were measured in plasma as markers for the antioxidative status once a week during the study period. To introduce an increased oxidative stress, a fish oil supplementation was given. The levels of alpha-tocopherol and ascorbic acid and the redox status did not change upon CoQ10 supplementation, while the level of TBARS decreased. The decrease in TBARS might be ascribed to an antioxidative effect of the supplied CoQ10. The constant redox level of CoQ10 during the CoQ10 supplementation shows that the exogenous CoQ10 is reduced during absorption and subsequent incorporation into lipoproteins, which is a prerequisite for its antioxidative function. The fish oil supplementation resulted in a higher TBARS level and a lower alpha-tocopherol level, but the redox level of CoQ10 was unchanged. In conclusion, the CoQ10 supplementation resulted in a higher plasma level of reduced CoQ10 and a lower TBARS level, but sparing of other plasma antioxidants (i.e. ascorbic acid and alpha-tocopherol) was not observed.
开展了一项纳入22名志愿者的人体研究，以调查膳食补充辅酶Q10对血液的抗氧化作用。在研究期间，每周一次测量血浆中α-生育酚、抗坏血酸、脂质过氧化（以硫代巴比妥酸反应物衡量）以及辅酶Q10的氧化还原状态（还原型辅酶Q10/总辅酶Q10），作为抗氧化状态的标志物。为引入增加的氧化应激，给予鱼油补充剂。补充辅酶Q10后，α-生育酚和抗坏血酸水平以及氧化还原状态未发生变化，而硫代巴比妥酸反应物水平降低。硫代巴比妥酸反应物水平的降低可能归因于所补充辅酶Q10的抗氧化作用。补充辅酶Q10期间辅酶Q10的氧化还原水平恒定，表明外源性辅酶Q10在吸收过程中被还原，随后并入脂蛋白，这是其抗氧化功能的一个先决条件。补充鱼油导致硫代巴比妥酸反应物水平升高和α-生育酚水平降低，但辅酶Q10的氧化还原水平未变。总之，补充辅酶Q10导致血浆中还原型辅酶Q10水平升高和硫代巴比妥酸反应物水平降低，但未观察到对其他血浆抗氧化剂（即抗坏血酸和α-生育酚）的节约作用。
