Cirilli Ilenia, Orlando Patrick, Hrelia Silvana, Marcheggiani Fabio, Tiano Luca, Beghelli Daniela, Angeloni Cristina
Department of Clinical Sciences, Section of Biochemistry, Polytechnic University of Marche, 60131, Ancona, Italy.
Department of Life and Environmental Sciences, Polytechnic University of Marche, 60131, Ancona, Italy.
Heliyon. 2024 Sep 12;10(18):e37854. doi: 10.1016/j.heliyon.2024.e37854. eCollection 2024 Sep 30.
Development and aging significantly impact the cellular levels of Coenzyme Q (CoQ), which is associated with both pathological and physiological conditions. Aim of this study was to describe the CoQ status throughout the lifetime of , a well-established model in aging studies. CoQ and CoQ distribution was analysed across different body segments and various life stages in both male and female flies. The results indicate that CoQ is the predominant isoform in every phase of flies' life cycle, with the highest concentrations observed in the thorax. We noted distinct trends in CoQ distribution during aging, which varied according to sex and body segments (head, thorax, and abdomen). Supplementation with two concentrations of CoQ and CoQ (15 μM and 75 μM) for 2 weeks induced a segment- and sex-specific CoQ uptake. Although 75 μM CoQ was more effective in modulating the CoQ status, lifelong treatment with this concentration did not affect the longevity of the flies.
发育和衰老会显著影响辅酶Q（CoQ）的细胞水平，这与病理和生理状况都有关联。本研究的目的是描述在衰老研究中一个成熟的模型——果蝇一生的辅酶Q状态。分析了雄性和雌性果蝇不同身体部位以及不同生命阶段的辅酶Q和辅酶Q分布情况。结果表明，辅酶Q是果蝇生命周期各阶段的主要异构体，在胸部观察到的浓度最高。我们注意到衰老过程中辅酶Q分布存在明显趋势，其因性别和身体部位（头部、胸部和腹部）而异。用两种浓度的辅酶Q和辅酶Q（15μM和75μM）补充2周可诱导特定部位和性别的辅酶Q摄取。尽管75μM的辅酶Q在调节辅酶Q状态方面更有效，但用该浓度进行终身治疗并未影响果蝇的寿命。
