Prisco D, Filippini M, Francalanci I, Paniccia R, Gensini G F, Serneri G G
Clinica Medica I, University of Florence, Italy.
Metabolism. 1995 May;44(5):562-9. doi: 10.1016/0026-0495(95)90111-6.
Twenty healthy male volunteers were randomly assigned to receive either four 1-g capsules of n-3 polyunsaturated fatty acids (PUFA) ethyl esters or four 1-g capsules of olive oil (as placebo) for a period of 4 months, followed by a 3-month wash-out period. Fatty acids of platelet phospholipid fractions, platelet aggregation, and thromboxane B2 (TXB2) formation were analyzed at 0, 2, and 4 months of treatment and at 1, 2, and 3 months of wash-out. During n-3 PUFA supplementation, accumulations of eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic (DHA) acids were markedly increased after 2 months, with slight differences in further accumulation up to 4 months among the various phospholipid fractions. Significant decreases in platelet sensitivity to collagen, serum TXB2 levels, and urinary TXB2 metabolites were also observed following n-3 PUFA treatment. During the first and second month of wash-out, slight differences were observed in changes of various fatty acids among different phospholipid fractions, but after 3 months of wash-out, alterations were no longer detectable with respect to pretreatment values. After 3 months of wash-out, platelet function parameters also were returned to baseline. Thus, both platelet lipids and function are influenced by n-3 PUFA ethyl ester supplementation, and significant alterations are still detectable after 2 months of wash-out.
20名健康男性志愿者被随机分为两组，一组服用4粒1克的n-3多不饱和脂肪酸（PUFA）乙酯胶囊，另一组服用4粒1克的橄榄油胶囊（作为安慰剂），为期4个月，随后有3个月的洗脱期。在治疗的0、2和4个月以及洗脱期的1、2和3个月时，分析血小板磷脂组分的脂肪酸、血小板聚集和血栓素B2（TXB2）的形成。在补充n-3 PUFA期间，2个月后二十碳五烯酸（EPA）、二十二碳五烯酸（DPA）和二十二碳六烯酸（DHA）的积累显著增加，直至4个月，各磷脂组分的进一步积累略有差异。n-3 PUFA治疗后，还观察到血小板对胶原蛋白的敏感性、血清TXB2水平和尿TXB2代谢产物显著降低。在洗脱期的第一个月和第二个月，不同磷脂组分中各种脂肪酸的变化存在细微差异，但在洗脱3个月后，与治疗前值相比，变化不再可检测到。洗脱3个月后，血小板功能参数也恢复到基线水平。因此，n-3 PUFA乙酯补充剂会影响血小板脂质和功能，洗脱2个月后仍可检测到显著变化。
