Prisco D, Filippini M, Francalanci I, Paniccia R, Gensini G F, Abbate K, Neri Serneri G G
Istituto di Clinica Medica Generale e Cardiologia, University of Florence, Italy.
Am J Clin Nutr. 1996 Jun;63(6):925-32. doi: 10.1093/ajcn/63.6.925.
To characterize the time course of plasma and red blood cell (RBC) changes after n-3 polyunsaturated fatty acid (PUFA) supplementation, 20 healthy male volunteers were randomly assigned to receive either four 1-g capsules of n-3 PUFA ethyl esters or four 1-g capsules of olive oil (as placebo) for a period of 4 mo, followed by a 3-mo washout period. Fatty acids of plasma and RBC phospholipid fractions were analyzed at 0, 2, and 4 mo of treatment and at 1, 2, and 3 mo of washout. During n-3 PUFA supplementation, accumulations of eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic (DHA) acids were marked after 2 mo with differences among different fractions of plasma and RBCs in further accumulation up to 4 mo. During the first and second months of the washout, slight differences were observed in changes of various fatty acids among different phospholipid fractions, but after 3 mo of washout, only minor alterations were still detectable with respect to pretreatment values. These data confirm the complex relations among different fatty acid pools after n-3 PUFA supplementation.
为了描述补充n-3多不饱和脂肪酸（PUFA）后血浆和红细胞（RBC）变化的时间进程，20名健康男性志愿者被随机分配，分别服用四粒1克的n-3 PUFA乙酯胶囊或四粒1克的橄榄油胶囊（作为安慰剂），为期4个月，随后是3个月的洗脱期。在治疗的0、2和4个月以及洗脱的1、2和3个月时分析血浆和RBC磷脂组分的脂肪酸。在补充n-3 PUFA期间，2个月后二十碳五烯酸（EPA）、二十二碳五烯酸（DPA）和二十二碳六烯酸（DHA）的积累明显，直至4个月时，血浆和RBC不同组分在进一步积累方面存在差异。在洗脱的第一个月和第二个月，不同磷脂组分中各种脂肪酸的变化存在轻微差异，但在洗脱3个月后，相对于预处理值仅可检测到微小变化。这些数据证实了补充n-3 PUFA后不同脂肪酸池之间的复杂关系。
