Yadav M, Nurhayati Z A, Padmanathan A, Abdul Aziz Y, Norhanom A W
Department of Genetics & Cellular Biology, University of Malaya, Kuala Lumpur.
Med J Malaysia. 1995 Mar;50(1):64-71.
Specific human papillomavirus (HPV) types have been implicated in the development of cervical carcinoma worldwide. Novel molecular techniques have facilitated the detection and typing of HPV in cervical lesions. DNA preparations from a series of 23 histopathologically confirmed cervical carcinoma patients were analyzed by polymerase chain reaction (PCR) using degenerate primers for the presence of HPV DNA sequences. A total of 22 of 23 cases studied (95.7%) were found positive for HPV DNA sequences. Further studies by DNA hybridization with viral specific probe and restriction enzyme analysis demonstrated the presence of HPV 16 in 73.9% (17/23) and HPV 18 in 65.2% (15/23) of the cases examined. Interestingly, the uncommon HPV 31 and 33 were also found but with a lower percentage (16.9%). It was noted that HPV 16 frequency in the carcinoma increased with age but HPV 18 was evenly present at all ages investigated. We found that HPV was frequently associated with the majority of the cervical carcinomas, and in all but one case, oncogenic high risk HPV genotypes were present. We conclude that HPV infection of the genital tract has an important role in the development of the disease in Malaysia.
在全球范围内，特定的人乳头瘤病毒（HPV）类型与宫颈癌的发生有关。新型分子技术促进了宫颈病变中HPV的检测和分型。使用简并引物通过聚合酶链反应（PCR）对一系列23例经组织病理学确诊的宫颈癌患者的DNA样本进行分析，以检测HPV DNA序列的存在。在所研究的23例病例中，共有22例（95.7%）被发现HPV DNA序列呈阳性。通过与病毒特异性探针的DNA杂交和限制性酶切分析进一步研究表明，在73.9%（17/23）的检测病例中存在HPV 16，在65.2%（15/23）的病例中存在HPV 18。有趣的是，还发现了罕见的HPV 31和33，但比例较低（16.9%）。值得注意的是，癌组织中HPV 16的频率随年龄增加而升高，但HPV 18在所有研究年龄组中分布均匀。我们发现HPV与大多数宫颈癌密切相关，除1例病例外，所有病例均存在致癌性高危HPV基因型。我们得出结论，生殖道HPV感染在马来西亚宫颈癌的发生中起重要作用。
