Yoko-o T, Kato H, Matsui Y, Takenawa T, Toh-e A
Department of Plant Sciences, Graduate School of Science, University of Tokyo, Japan.
Mol Gen Genet. 1995 Apr 20;247(2):148-56. doi: 10.1007/BF00705644.
The PLC1 gene of the yeast Saccharomyces cerevisiae has been discovered to encode a homolog of mammalian phosphoinositide-specific phospholipase C (PLC). Five temperature-sensitive plc1 mutants were isolated by in vitro mutagenesis with subsequent plasmid shuffling. All of the amino acid substitutions that caused a temperature-sensitive growth phenotype were located in the X or the Y region, both of which are conserved among PLC isoenzymes. The PLC activity of all products of mutant plc1 genes was dramatically lower than that of the wild-type product, indicating that PLC activity itself is important for cell growth. At the restrictive temperature, plc1 mutant cells ceased growth at random times during the cell cycle, a result that suggests that PLC1 is required at several or all stages of the cell cycle.
已发现酿酒酵母的PLC1基因编码一种哺乳动物磷酸肌醇特异性磷脂酶C（PLC）的同源物。通过体外诱变和随后的质粒洗牌分离出五个温度敏感型plc1突变体。所有导致温度敏感型生长表型的氨基酸取代都位于X或Y区域，这两个区域在PLC同工酶中都是保守的。突变plc1基因的所有产物的PLC活性都显著低于野生型产物，表明PLC活性本身对细胞生长很重要。在限制温度下，plc1突变体细胞在细胞周期的随机时间停止生长，这一结果表明在细胞周期的几个或所有阶段都需要PLC1。
