酿酒酵母假定的磷酸肌醇特异性磷脂酶C基因PLC1对细胞生长很重要。

Yoko-o T, Matsui Y, Yagisawa H, Nojima H, Uno I, Toh-e A

Department of Biology, Faculty of Science, University of Tokyo, Japan.

Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1804-8. doi: 10.1073/pnas.90.5.1804.

Using the polymerase chain reaction technique, we have isolated a gene that encodes a putative phosphoinositide-specific phospholipase C (PLC) in the yeast Saccharomyces cerevisiae. The nucleotide sequence indicates that the gene encodes a polypeptide of 869 amino acid residues with a calculated molecular mass of 101 kDa. This polypeptide has both the X and Y regions conserved among mammalian PLC-beta, -gamma, and -delta, and the structure is most similar to that of mammalian PLC-delta. This putative yeast PLC gene has been designated PLC1. Disruption of PLC1 results in slow growth or lethality for cells, depending on their genetic background and the medium, indicating that PLC1 is important for cell growth. Expression of rat PLC-delta 1 cDNA suppressed the growth defect of plc1 disruptants, strongly suggesting that PLC1 encodes PLC.

利用聚合酶链反应技术，我们在酿酒酵母中分离出了一个编码假定的磷酸肌醇特异性磷脂酶C（PLC）的基因。核苷酸序列表明该基因编码一个由869个氨基酸残基组成的多肽，计算分子量为101 kDa。该多肽具有在哺乳动物PLC-β、-γ和-δ中保守的X和Y区域，其结构与哺乳动物PLC-δ最为相似。这个假定的酵母PLC基因被命名为PLC1。PLC1的破坏会导致细胞生长缓慢或致死，这取决于它们的遗传背景和培养基，表明PLC1对细胞生长很重要。大鼠PLC-δ1 cDNA的表达抑制了plc1破坏株的生长缺陷，强烈表明PLC1编码PLC。