黄热病病毒疫苗株17DD和17D - 213的完整核苷酸序列。

Complete nucleotide sequence of yellow fever virus vaccine strains 17DD and 17D-213.

作者信息

dos Santos C N, Post P R, Carvalho R, Ferreira I I, Rice C M, Galler R

机构信息

Fundação Oswaldo Cruz, Departamento de Bioquimica e Biologia Molecular, Rio de Janeiro, Brazil.

出版信息

Virus Res. 1995 Jan;35(1):35-41. doi: 10.1016/0168-1702(94)00076-o.

DOI:10.1016/0168-1702(94)00076-o

PMID:7754673

Abstract

The complete nucleotide sequence of the genome from two yellow fever (YF) virus vaccine strains, 17DD and 17D-213, has been determined. Comparison of these sequences with those of other YF viruses including the parental virulent Asibi strain allowed the identification of 48 nucleotide sequence differences which are common to all 17D substrains. This is a significant reduction from the 67 nucleotide changes originally reported as being 17D-specific and potentially related to viral attenuation. The 48 changes are scattered throughout the genome, 26 of which are silent and 22 led to amino acid substitutions. These 22 changes are bona fide candidates to test by mutating the infectious YF cDNA to investigate their role in viral attenuation.

摘要

已确定两种黄热病（YF）病毒疫苗株17DD和17D - 213的基因组完整核苷酸序列。将这些序列与包括亲本强毒株阿西比株在内的其他YF病毒的序列进行比较，鉴定出了48个核苷酸序列差异，这些差异是所有17D亚株共有的。这与最初报道的17D特异性且可能与病毒减毒有关的67个核苷酸变化相比有显著减少。这48个变化分散在整个基因组中，其中26个是沉默突变，22个导致氨基酸替换。通过将感染性YF cDNA进行突变来测试这22个变化，以研究它们在病毒减毒中的作用，它们是真正的候选对象。