黄热病病毒17D亚株间的遗传变异性。
Genetic variability among yellow fever virus 17D substrains.
作者信息
Galler R, Post P R, Santos C N, Ferreira I I
机构信息
Departamento de Bioquimica e Biologia Molecular, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
出版信息
Vaccine. 1998 May-Jun;16(9-10):1024-8. doi: 10.1016/s0264-410x(97)00278-8.
The complete nucleotide sequence of the genome from two yellow fever (YF) virus strains, 17DD and 17D-213 was determined. Comparison of these sequences with those of other YF viruses, including the parental virulent Asibi strain, allowed the identification of 48 nucleotide sequence differences which are 17D strain-specific and potentially related to viral attenuation. Another 43 nucleotide sequence differences were not common to all 17D substrains and are therefore substrain specific. Of the 21 changes between 17DD and Asibi 15 only five led to amino acid substitutions whereas 13 substrain differences common to all 17D-204 substrains produced six amino acid substitutions. Since the exact passage histories of these viruses is known it was possible to calculate, for each strain, the number of accumulated changes per passage. Based on these data the 17DD strain was the most genetically stable virus.
测定了两种黄热病（YF）病毒株17DD和17D - 213的基因组完整核苷酸序列。将这些序列与其他黄热病病毒的序列进行比较，包括亲本强毒株阿西比株，鉴定出48个核苷酸序列差异，这些差异是17D株特有的，可能与病毒减毒有关。另外43个核苷酸序列差异并非所有17D亚株共有，因此是亚株特异性的。在17DD和阿西比之间的21个变化中，只有5个导致氨基酸替换，而所有17D - 204亚株共有的13个亚株差异产生了6个氨基酸替换。由于这些病毒的确切传代历史已知，因此有可能计算出每种毒株每代积累的变化数。基于这些数据，17DD株是遗传上最稳定的病毒。
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