Manowitz P, Stoecker P W, Yacynych A M
UMDNJ-Robert Wood Johnson Medical School, Department of Psychiatry, Piscataway 08854, USA.
Biosens Bioelectron. 1995;10(3-4):359-70. doi: 10.1016/0956-5663(95)96854-r.
A biosensor using a composite polymer to prevent interferences was used in a flow injection analysis system for the detection of galactose in human plasma. The biosensor consisted of galactose oxidase immobilized on a platinized carbon electrode that had been modified with a composite polymer. The composite polymer showed improved selectivity to hydrogen peroxide compared with either of its individual polymeric components, Nafion and a copolymer of diaminobenzene and resorcinol. The composite polymer minimized the effect of possible interference from urate, ascorbate, and acetaminophen. This analytical system had a minimum detection limit of 50 microM, linearity to 6 mM, a storage stability of greater than 30 days, and a high sample throughput (approx. 120 samples/h).
一种使用复合聚合物来防止干扰的生物传感器被应用于流动注射分析系统中，用于检测人血浆中的半乳糖。该生物传感器由固定在经复合聚合物修饰的镀铂碳电极上的半乳糖氧化酶组成。与它的两种单独的聚合物成分（Nafion以及二氨基苯和间苯二酚的共聚物）相比，这种复合聚合物对过氧化氢表现出更高的选择性。该复合聚合物将尿酸盐、抗坏血酸盐和对乙酰氨基酚可能产生的干扰影响降至最低。这个分析系统的最低检测限为50微摩尔，线性范围至6毫摩尔，储存稳定性大于30天，并且具有高样品通量（约120个样品/小时）。
