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采用酶联免疫吸附测定法对美国1993年小麦和大麦作物中的脱氧雪腐镰刀菌烯醇进行检测。

Survey of deoxynivalenol in U.S. 1993 wheat and barley crops by enzyme-linked immunosorbent assay.

作者信息

Trucksess M W, Thomas F, Young K, Stack M E, Fulgueras W J, Page S W

机构信息

U.S. Food and Drug Administration, Division of Natural Products, Washington, DC 20204, USA.

出版信息

J AOAC Int. 1995 May-Jun;78(3):631-6.

PMID:7756875
Abstract

Wheat and barley from the 1993 crop year were analyzed for deoxynivalenol (DON). A total of 630 samples were collected by the Federal Grain Inspection Service in 25 states and analyzed using a commercially available, direct competitive, enzyme-linked immunosorbent assay. The limit of determination was about 0.5 micrograms/g. DON contamination in the 483 wheat samples averaged 2.0 micrograms/g and ranged from < 0.5 to 18 micrograms/g. DON contamination in the 147 barley samples averaged 4.2 micrograms/g and ranged from < 0.5 to 26 micrograms/g. About 40% fo the wheat samples and 57% of the barley samples contained DON levels that were greater than the U.S. Food and Drug Administration 1982 advisory level of 2 micrograms/g for DON in wheat designated for milling (human consumption).

摘要

对1993作物年度的小麦和大麦进行了脱氧雪腐镰刀菌烯醇(DON)分析。联邦谷物检验局在25个州共采集了630个样本,并使用一种市售的直接竞争酶联免疫吸附测定法进行分析。测定限约为0.5微克/克。483个小麦样本中的DON污染平均为2.0微克/克,范围为<0.5至18微克/克。147个大麦样本中的DON污染平均为4.2微克/克,范围为<0.5至26微克/克。约40%的小麦样本和57%的大麦样本中DON含量高于美国食品药品监督管理局1982年针对用于制粉(供人类食用)的小麦中DON设定的2微克/克的建议水平。

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