用于从特定高水分食品中分离沙门氏菌的选择性平板琼脂的相对有效性。

Relative effectiveness of selective plating agars for recovery of Salmonella species from selected high-moisture foods.

作者信息

Sherrod P S, Amaguana R M, Andrews W H, June G A, Hammack T S

机构信息

U.S. Food and Drug Administration, Division of Microbiological Studies, Washington, DC 20204, USA.

出版信息

J AOAC Int. 1995 May-Jun;78(3):679-90.

PMID:7756882

Abstract

The relative effectiveness of 6 selective plating media were compared for effectiveness in recovery of Salmonella spp. from selected high-moisture foods. Three new plating agars (EF-18, Rambach, and xylose lysine Tergitol-4) and 3 selective plating agars (bismuth sulfite, Hektoen enteric, and xylose lysine desoxycholate) recommended by AOAC INTERNATIONAL and the Bacteriological Analytical Manual (BAM) were compared. The agars were streaked from cultures selectively enriched in selenite cystine broth, tetrathionate broth, and Rappaport-Vassiliadis medium. The high-moisture foods studied were naturally contaminated pork sausage, chicken parts, turkey parts, and frog legs and artificially contaminated shrimp, oysters, egg yolks, and lettuce. The relative effectiveness of each selective plating agar was determined by recovery of Salmonella spp. and enumeration of false-positive and false-negative reactions. Although the new selective plating agars compared favorably with the AOAC/BAM-recommended agars, they offered no advantage. Incubation of selective enrichment broths at elevated temperatures decreased the numbers of false-positive and false-negative reactions for all 6 selective plating agars.

摘要

比较了6种选择性平板培养基从特定高水分食品中回收沙门氏菌的相对有效性。比较了三种新的平板琼脂（EF-18、兰巴赫琼脂和木糖赖氨酸胆盐-4）以及美国公职分析化学家协会（AOAC INTERNATIONAL）和《细菌学分析手册》（BAM）推荐的三种选择性平板琼脂（亚硫酸铋琼脂、赫克托恩肠道琼脂和木糖赖氨酸脱氧胆酸盐琼脂）。这些琼脂是从在亚硒酸盐胱氨酸肉汤、四硫磺酸盐肉汤和拉帕波特-瓦西利亚迪斯培养基中选择性富集的培养物中划线接种的。所研究的高水分食品包括天然污染的猪肉香肠、鸡肉块、火鸡块和蛙腿，以及人工污染的虾、牡蛎、蛋黄和生菜。通过回收沙门氏菌以及对假阳性和假阴性反应进行计数来确定每种选择性平板琼脂的相对有效性。尽管新的选择性平板琼脂与AOAC/BAM推荐的琼脂相比表现良好，但它们并无优势。在较高温度下孵育选择性富集肉汤可减少所有6种选择性平板琼脂的假阳性和假阴性反应数量。