Andrews W H, Wilson C R, Poelma P L, Romero A
Appl Environ Microbiol. 1977 Jan;33(1):65-8. doi: 10.1128/aem.33.1.65-68.1977.
Four methods of sample preparation were compared for their relative efficiency in recovering Salmonella from imported frog legs. No significant difference (P greater than 0.10) was observed in the efficiency of submersion, blending, and stomaching methods, but rinsing recovered significantly fewer (P less than 0.01) Salmonella-positive frog legs than the other three methods. No significant difference (P less than 0.25) was observed in the number of positive frog legs recovered by selenite cystine or tetrathionate broth. Salmonella-Shigella agar, when streaked from either of these broths, gave significantly fewer (P less than 0.01) Salmonella-positive frog legs than brilliant green, bismuth sulfite, xylose lysine deoxycholate, and Hektoen-enteric agars. Use selective agars, resulted in detection of an additional 11 and 6 Salmonella-positive frog legs, respectively. A variety of serotypes, mostly uncommon, was recovered. One Salmonella serotype (6,14,24:r,i:e,n,z15), possessing a heretofore unreported antigenic formula, was isolated.
比较了四种样品制备方法从进口蛙腿中回收沙门氏菌的相对效率。浸没、搅拌和捣碎法的效率未观察到显著差异（P大于0.10），但冲洗法回收的沙门氏菌阳性蛙腿明显少于其他三种方法（P小于0.01）。亚硒酸盐胱氨酸肉汤或四硫磺酸盐肉汤回收的阳性蛙腿数量未观察到显著差异（P小于0.25）。从这些肉汤中的任何一种划线接种沙门氏菌-志贺氏菌琼脂，得到的沙门氏菌阳性蛙腿明显少于亮绿琼脂、亚硫酸铋琼脂、木糖赖氨酸脱氧胆酸盐琼脂和赫氏肠道琼脂（P小于约0.01）。使用选择性琼脂分别额外检测到11个和6个沙门氏菌阳性蛙腿。回收了多种血清型，大多不常见。分离出一种沙门氏菌血清型（6,14,24:r,i:e,n,z15），其具有迄今未报道的抗原公式。
