Togashi K, Ando K, Kameya T, Kawakami M
Kitasato Biochemical Laboratories SMI-Bristol Ltd., Sagamihara.
Rinsho Byori. 1991 Mar;39(3):283-8.
We established specific and highly sensitive radioimmunoassay (RIA) for the detection of human brain natriuretic peptide (BNP), and confirmed the presence of BNP-like immunoreactivity (BNP-LI) in human circulating blood and the heart. The antibody used in this RIA did not react any known human atrial natriuretic peptide (ANP). Gel permeation chromatography coupled with the RIA revealed that BNP-LI in atrium is composed with high molecular BNP and low molecular BNP. Furthermore, dominant form of BNP-LI in human plasma was thought to be low molecular BNP. Mean concentration of BNP-LI in plasma from normal subjects was 5.9 pg/ml, which was about 5 times lower than that of ANP-LI. Mean concentration of BNP-LI in human cardiac atrium was 45.6 ng/mg protein, which was about 40-fold lower than that of ANP-LI. Furthermore, concentration of BNP-LI in human cardiac ventricle was in a range of 0.1-4.8 ng/mg protein, being about 1/20 that of cardiac atrium. These data suggest that BNP synthesized in human heart and secreted into circulation.
我们建立了用于检测人脑钠肽（BNP）的特异性高灵敏度放射免疫分析（RIA）方法，并证实人循环血液和心脏中存在BNP样免疫反应性（BNP-LI）。该RIA中使用的抗体与任何已知的人心房钠尿肽（ANP）均无反应。凝胶渗透色谱结合RIA显示，心房中的BNP-LI由高分子量BNP和低分子量BNP组成。此外，人血浆中BNP-LI的主要形式被认为是低分子量BNP。正常受试者血浆中BNP-LI的平均浓度为5.9 pg/ml，约为ANP-LI的1/5。人心脏心房中BNP-LI的平均浓度为45.6 ng/mg蛋白质，约为ANP-LI的1/40。此外，人心脏心室中BNP-LI的浓度在0.1-4.8 ng/mg蛋白质范围内，约为心脏心房的1/20。这些数据表明，BNP在人心脏中合成并分泌入循环。
