Röpke E M, Augustin W, Gollnick H
Department of Dermatology, Medical Faculty, Otto von Guericke University Magdeburg, Germany.
Skin Pharmacol. 1996;9(6):381-7. doi: 10.1159/000211449.
A non-invasive method for sampling skin surface lipids is cyanoacrylate stripping (CAC-TS). It was the purpose of our study to improve the method for sampling of skin surface lipids and the separation of epidermal lipid fractions by a modification of the methods described by Melnik and by Imokawa et al. Briefly, lipids on the glass slide sampled by CAC-TS from the forearm of 75 volunteers and from the forehead of 60 volunteers were eluted in hexane/ethanol under ultrasonication. Identification of the diluted total superficial sebaceous and epidermal skin lipids was performed by sequential high-performance thin-layer chromatography. For quantification of the lipids we used densitometric methods. By this modified method we were able to show a clear and complete separation of all relevant lipids from a cyanoacrylate strip that represented 1-2 mg stratum corneum only.
一种用于采集皮肤表面脂质的非侵入性方法是氰基丙烯酸酯剥离法（CAC - TS）。我们研究的目的是通过改进梅尔尼克以及井川等人所描述的方法，来完善皮肤表面脂质的采集方法以及表皮脂质组分的分离方法。简而言之，通过CAC - TS从75名志愿者的前臂以及60名志愿者的前额采集到玻片上的脂质，在超声处理下用己烷/乙醇洗脱。通过连续高效薄层色谱法对稀释后的总浅表皮脂腺和表皮皮肤脂质进行鉴定。我们使用光密度测定法对脂质进行定量。通过这种改进方法，我们能够从仅代表1 - 2毫克角质层的氰基丙烯酸酯条带上清晰且完全地分离出所有相关脂质。
