Gomes R F, Macedo A M, Pena S D, Melo M N
Departamento de Parasitologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Brazil.
Exp Parasitol. 1995 Jun;80(4):681-7. doi: 10.1006/expr.1995.1084.
Leishmania (Viannia) braziliensis isolates from two different geographic areas in Brazil were studied by DNA fingerprinting with the 33.15 multilocal probe and PCR with arbitrary primers (random amplification of polymorphic DNA-RAPD). The genetic distance of strains was measured by band sharing. The results showed that the strains isolated in Minas Gerais, southeastern Brazil, are very different from those isolated in Pará, northern Brazil. Strains from Minas Gerais constituted a relatively homogeneous group, presenting DNA fingerprint patterns with 76% of shared bands and RAPD profiles with 93% of shared bands. In contrast, strains from Pará showed higher genetic variability, with only 17 and 45% of shared bands in DNA fingerprint and RAPD patterns, respectively. This study suggests that genetic differences between L. braziliensis from both areas might have an epidemiological significance.
利用33.15多区域探针进行DNA指纹分析以及使用任意引物进行PCR（随机扩增多态性DNA - RAPD），对来自巴西两个不同地理区域的巴西利什曼原虫（维安尼亚亚属）分离株进行了研究。通过条带共享来测量菌株的遗传距离。结果显示，巴西东南部米纳斯吉拉斯州分离出的菌株与巴西北部帕拉州分离出的菌株差异很大。来自米纳斯吉拉斯州的菌株构成了一个相对同质的群体，其DNA指纹图谱有76%的条带共享，RAPD图谱有93%的条带共享。相比之下，来自帕拉州的菌株表现出更高的遗传变异性，在DNA指纹图谱和RAPD图谱中分别只有17%和45%的条带共享。这项研究表明，这两个地区的巴西利什曼原虫之间的遗传差异可能具有流行病学意义。
