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使用常规监测样本对三种抗生素微生物筛选方法的比较。

Comparison of three microbial screening methods for antibiotics using routine monitoring samples.

作者信息

Pikkemaat Mariël G, Rapallini Michel L B A, Dijk Sabrina Oostra-van, Elferink J W Alexander

机构信息

RIKILT, Institute of Food Safety, Wageningen University and Research Centre, P.O. Box 230, 6700 AE Wageningen, The Netherlands.

出版信息

Anal Chim Acta. 2009 Apr 1;637(1-2):298-304. doi: 10.1016/j.aca.2008.08.023. Epub 2008 Aug 28.

Abstract

Monitoring large numbers of slaughter animals for the presence of antimicrobial residues is preferably carried out using microbiological screening methods, because of their high cost-effectiveness. An evaluation of the Nouws antibiotic test (NAT) was performed on routine monitoring samples and the performance of the method was compared with two other microbial screening methods: Screening test for antibiotic residues (STAR) and Premi Test. Analysis of 591 samples yielded four MRL violations. Three of them concerned tetracyclines that were only detected with the NAT and the STAR method. The fourth, 172 microgkg(-1) Sulfadiazine, was detected by all three methods. Additionally, 156 microgkg(-1) Tulathromycin was found in porcine meat, while for this residue no MRL in muscle has been established.

摘要

由于微生物筛选方法具有较高的成本效益,因此监测大量屠宰动物体内抗菌药物残留情况时,最好采用此类方法。对常规监测样本进行了努斯抗生素检测(NAT)评估,并将该方法的性能与其他两种微生物筛选方法进行了比较:抗生素残留筛选检测(STAR)和普瑞米检测。对591份样本的分析发现了4例违反最大残留限量(MRL)的情况。其中3例涉及四环素,仅通过NAT和STAR方法检测到。第四例,172微克/千克磺胺嘧啶,三种方法均检测到。此外,在猪肉中发现了156微克/千克的泰拉霉素,而肌肉中该残留物尚无最大残留限量规定。

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