一种用于检测拉沙洛西的酶联免疫吸附测定法的开发及家禽体内拉沙洛西残留的消除动力学研究

Development of an ELISA for lasalocid and depletion kinetics of lasalocid residues in poultry.

作者信息

Kennedy D G, Blanchflower W J, O'Dornan B C

机构信息

Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast, UK.

出版信息

Food Addit Contam. 1995 Jan-Feb;12(1):83-92. doi: 10.1080/02652039509374282.

DOI:10.1080/02652039509374282

PMID:7758635

Abstract

Polyclonal antibodies against the coccidiostat, lasalocid, were raised in sheep. The antisera were applied in an enzyme-linked immunosorbent assay (ELISA) for lasalocid, which was validated for chicken serum, liver and muscle. Bridge homology in the ELISA was overcome by absorbing unspecific antisera onto a conjugate between salinomycin and chicken serum albumin, which was immobilized onto Biosilon beads. The described assay is highly specific for lasalocid, and is capable of detecting lasalocid at concentrations of less than 0.15 ng/g, depending on the tissue. Residual concentrations of lasalocid in broilers fed medicated feed containing 90 mg/kg lasalocid were measured during a 7 day withdrawal period. The half-life of lasalocid was 11, 36 and 41 h in serum, liver and muscle, respectively. Lasalocid concentrations in liver were approximately 10 ng/g 7 days withdrawal of the medicated feed.

摘要

针对抗球虫药拉沙洛西钠制备了多克隆抗体，所用动物为绵羊。将抗血清应用于拉沙洛西钠的酶联免疫吸附测定（ELISA），该方法已在鸡血清、肝脏和肌肉中得到验证。通过将非特异性抗血清吸附到盐霉素与鸡血清白蛋白的偶联物上，克服了ELISA中的交叉同源性问题，该偶联物固定在Biosilon珠上。所述测定方法对拉沙洛西钠具有高度特异性，根据组织不同，能够检测浓度低于0.15 ng/g的拉沙洛西钠。在7天的停药期内，测定了喂食含90 mg/kg拉沙洛西钠药物饲料的肉鸡体内拉沙洛西钠的残留浓度。拉沙洛西钠在血清、肝脏和肌肉中的半衰期分别为11、36和41小时。停药7天后，肝脏中拉沙洛西钠的浓度约为10 ng/g。