Kennedy D G, Blanchflower W J, O'Dornan B C
Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast, UK.
Food Addit Contam. 1995 Jan-Feb;12(1):93-9. doi: 10.1080/02652039509374283.
Salinomycin is widely used as a feed additive to control coccidiosis in the broiler industry world-wide. EC legislation on veterinary drug residues will soon be extended to cover poultry. This will require the development of rapid assays to screen for the presence of residual concentrations of this and other drugs in poultry meat. This study describes the development of an enzyme-linked immunosorbent assay for salinomycin that has a limit of detection of approximately 0.2 ng/g. The antibody cross-reacts with narasin, but is not subject to interference from lasalocid, maduramicin or monensin. Residual concentrations of salinomycin were measured in the tissues of broilers following feeding of medicated feed containing 60 mg/kg salinomycin. Salinomycin residues were present only at very low concentrations in liver and muscle, and fell below the limit of decision of the assay within 2 days of withdrawal of the medicated feed.
盐霉素在全球肉鸡养殖业中被广泛用作饲料添加剂以控制球虫病。欧盟关于兽药残留的法规不久将扩展至涵盖家禽。这将需要开发快速检测方法，以筛查禽肉中这种及其他药物的残留浓度。本研究描述了一种盐霉素酶联免疫吸附测定法的开发，其检测限约为0.2纳克/克。该抗体与那拉菌素发生交叉反应，但不受拉沙洛西、马杜霉素或莫能菌素的干扰。在给肉鸡投喂含60毫克/千克盐霉素的加药饲料后，测定了其组织中的盐霉素残留浓度。盐霉素残留仅在肝脏和肌肉中以极低浓度存在，且在停用加药饲料后2天内降至该测定法的判定限以下。
