Sukcharoen N
Department of Obstetrics and Gynaecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
J Med Assoc Thai. 1994 Nov;77(11):605-11.
To determine the improvement of sperm movement characteristics after discontinuous Percoll gradients preparation, sperm movement characteristics from 60 normal semen samples were assessed before and after discontinuous Percoll gradients preparation using Hamilton-Thorn Motility Analyzer (HTMA). There was a significant correlation between manual and HTMA results for the percentage of motile sperm (r = 0.8670, p < 0.001). The percentage rapid, percentage motile, average path velocity (VAP), progressive velocity (VSL), curvilinear velocity (VCL), beat cross frequency (BCF), amplitude of head displacement (ALH), percentage of progressive motility, and hyperactivation were significantly increased after preparation. Therefore, this sperm preparation technique is a convenient and efficient method to prepare progressive motile sperm for assisted conceptive technology.
为了确定不连续 Percoll 梯度制备后精子运动特征的改善情况，使用汉密尔顿-桑恩运动分析仪（HTMA）对 60 份正常精液样本在不连续 Percoll 梯度制备前后的精子运动特征进行了评估。活动精子百分比的人工检测结果与 HTMA 结果之间存在显著相关性（r = 0.8670，p < 0.001）。制备后，快速运动百分比、活动百分比、平均路径速度（VAP）、直线速度（VSL）、曲线速度（VCL）、鞭打交叉频率（BCF）、头部位移幅度（ALH）、进行性运动百分比和超激活均显著增加。因此，这种精子制备技术是一种为辅助生殖技术制备进行性运动精子的便捷高效方法。
