Sukcharoen N
Department of Obstetrics and Gynaecology, Faculty of Medicine, Chulalongkorn University, Bangkok Thailand.
J Med Assoc Thai. 1995 Jan;78(1):22-9.
To determine the improvement of sperm morphology and sperm nuclear DNA normality after discontinuous Percoll gradients preparation, sperm morphology and sperm nuclear DNA normality from 157 semen samples from 45 donors and 63 male partners of infertile couples were assessed before and after discontinuous Percoll gradients preparation. In semen samples, about 67.5 per cent of the nuclei exhibited green acridine orange fluorescence. The percentage of normal sperm morphology, percentage of progressive motility, and percentage of green-fluorescing sperm were significantly better after this sperm preparation. Therefore, this sperm preparation technique is a convenient and efficient method to select progressive motile, normal morphological and normal nuclear DNA sperm for assisted conceptive technology.
为了确定不连续Percoll梯度制备后精子形态及精子核DNA正常率的改善情况，对45名供者和63名不育夫妇男性伴侣的157份精液样本在不连续Percoll梯度制备前后的精子形态及精子核DNA正常率进行了评估。在精液样本中，约67.5%的细胞核呈现绿色吖啶橙荧光。经过这种精子制备后，正常精子形态百分比、前向运动百分比和绿色荧光精子百分比均有显著改善。因此，这种精子制备技术是一种为辅助生殖技术选择具有前向运动能力、形态正常且核DNA正常的精子的便捷高效方法。
