Recio M C, Just M J, Giner R M, Mañez S, Rios J L, Hostettmann K
Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Spain.
J Nat Prod. 1995 Jan;58(1):140-4. doi: 10.1021/np50115a023.
By means of activity-directed chromatographic fractionation using the 12-O-tetradecanoylphorbol acetate (TPA)-induced edema test, two saikosaponins were isolated from the MeOH extract of Heteromorpha trifoliata leaves. They were identified as 16 beta, 23-dihydroxy-13,28-epoxyolean-11-en-3 beta-yl-[beta-D-glucopyranosyl (1-->2)]-[beta-D-glucopyranosyl (1-->3)]-beta-D-fucopyranoside [1] and 16 beta, 23,28-trihydroxy-11 alpha-methoxyolean-12-en-3 beta-yl-[beta-D-glucopyranosyl (1-->2)]-[beta-D-glucopyranosyl (1-->3) [beta-D-fucopyranoside [2]. Compound 1 showed activity in the TPA and ethylphenylpropiolate (EPP) mouse ear edema and the serotonin paw edema tests, whereas compound 2 was active only in the mouse ear edema model. Both substances had only a slight effect against a carrageenan paw edema model. The anti-inflammatory action of compound 1 was notably decreased by the mRNA and protein synthesis inhibitors actinomycin D and cycloheximide.
通过使用12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）诱导的水肿试验进行活性导向色谱分离，从异叶山绿豆叶的甲醇提取物中分离出两种柴胡皂苷。它们被鉴定为16β，23 - 二羟基 - 13,28 - 环氧齐墩果 - 11 - 烯 - 3β - 基 - [β - D - 吡喃葡萄糖基（1→2）] - [β - D - 吡喃葡萄糖基（1→3）] - β - D - 岩藻糖苷[1]和16β，23,28 - 三羟基 - 11α - 甲氧基齐墩果 - 12 - 烯 - 3β - 基 - [β - D - 吡喃葡萄糖基（1→2）] - [β - D - 吡喃葡萄糖基（1→3）][β - D - 岩藻糖苷[2]。化合物1在TPA和乙基苯基丙炔酸酯（EPP）诱导的小鼠耳部水肿以及血清素诱导的爪部水肿试验中显示出活性，而化合物2仅在小鼠耳部水肿模型中具有活性。两种物质对角叉菜胶诱导的爪部水肿模型仅有轻微作用。化合物1的抗炎作用被mRNA和蛋白质合成抑制剂放线菌素D和环己酰亚胺显著降低。
