Li E, Clark A M, Rotella D P, Hufford C D
Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University 38677.
J Nat Prod. 1995 Jan;58(1):74-81. doi: 10.1021/np50115a009.
Ophiobolin A [1], 3-anhydroophiobolin A [2], ophiobolin B [3], and ophiobolin L [4] were isolated from fermentation broths of Cochliobolus heterostrophus. Preliminary screening showed that a number of organisms were capable of metabolizing the sesterterpene ophiobolin A [1]. Large-scale transformations of ophiobolin A [1] with Polyangium cellulosum produced 6 and 7 while Pseudomonas aeruginosa produced 8. Resting-cell preparations of Penicillium patulum afforded 9 and 10. The structures of these metabolites were established by spectroscopic methods and by comparison of the spectral data with those of the starting material. The antimicrobial activity of the ophiobolins was also evaluated.
蛇孢菌素A [1]、3-脱水蛇孢菌素A [2]、蛇孢菌素B [3]和蛇孢菌素L [4]是从玉米大斑病菌的发酵液中分离得到的。初步筛选表明，许多生物体能够代谢倍半萜烯蛇孢菌素A [1]。用纤维堆囊菌对蛇孢菌素A [1]进行大规模转化得到了化合物6和7，而铜绿假单胞菌则产生了化合物8。展青霉的静息细胞制剂得到了化合物9和10。这些代谢产物的结构通过光谱方法以及将光谱数据与起始原料的数据进行比较得以确定。还评估了蛇孢菌素的抗菌活性。
