Suzuki E, Guo K, Kolman M, Yu Y T, Walsh K
Division of Cardiovascular Research, St. Elizabeth's Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02135, USA.
Mol Cell Biol. 1995 Jun;15(6):3415-23. doi: 10.1128/MCB.15.6.3415.
Vascular smooth muscle cells (VSMCs) reversibly coordinate the expression of VSMC-specific genes and the genes required for cell cycle progression. Here we demonstrate that isoforms of the MEF2/RSRF transcription factor are expressed in VSMCs and in vascular tissue. The MEF2A DNA-binding activity was upregulated when quiescent VSMCs were stimulated to proliferate with serum mitogens. The serum-induction of MEF2A DNA-binding activity occurred approximately 4 h following serum activation, and this correlated with an increase in the level of MEF2A protein without changes in the level of MEF2A mRNA or protein stability. These results indicate that MEF2A induction by serum is regulated at the level of translation.
血管平滑肌细胞（VSMC）可逆地协调VSMC特异性基因和细胞周期进程所需基因的表达。在此，我们证明MEF2/RSRF转录因子的异构体在VSMC和血管组织中表达。当静止的VSMC被血清促有丝分裂原刺激增殖时，MEF2A的DNA结合活性上调。血清对MEF2A DNA结合活性的诱导在血清激活后约4小时出现，这与MEF2A蛋白水平的增加相关，而MEF2A mRNA水平或蛋白稳定性没有变化。这些结果表明血清对MEF2A的诱导是在翻译水平上受到调控的。
