Cai W, Aburatani H, Stanton V P, Housman D E, Wang Y K, Schwartz D C
W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, NY 10003, USA.
Proc Natl Acad Sci U S A. 1995 May 23;92(11):5164-8. doi: 10.1073/pnas.92.11.5164.
We have developed a surface mounting technology for the rapid construction of ordered restriction maps from individual DNA molecules. Optical restriction maps constructed from yeast artificial chromosome DNA molecules mounted on specially derivatized glass surfaces are accurate and reproducible, and the technology is amenable to automation. The mounting procedures described here should also be useful for fluorescence in situ hybridization studies. We believe these improvements to optical mapping will further stimulate the development of nonelectrophoretic approaches to genome analysis.
我们已经开发出一种表面安装技术，用于从单个DNA分子快速构建有序限制图谱。从安装在经过特殊衍生化处理的玻璃表面上的酵母人工染色体DNA分子构建的光学限制图谱准确且可重复，并且该技术适合自动化操作。这里描述的安装程序对于荧光原位杂交研究也应该是有用的。我们相信这些对光学图谱的改进将进一步推动非电泳基因组分析方法的发展。
