酿酒酵母基因组110千碱基分辨率的物理图谱。
Physical map of the Saccharomyces cerevisiae genome at 110-kilobase resolution.
作者信息
Link A J, Olson M V
机构信息
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110.
出版信息
Genetics. 1991 Apr;127(4):681-98. doi: 10.1093/genetics/127.4.681.
Abstract
A physical map of the Saccharomyces cerevisiae genome is presented. It was derived by mapping the sites for two restriction endonucleases, SfiI and NotI, each of which recognizes an 8-bp sequence. DNA-DNA hybridization probes for genetically mapped genes and probes that span particular SfiI and NotI sites were used to construct a map that contains 131 physical landmarks--32 chromosome ends, 61 SfiI sites and 38 NotI sites. These landmarks are distributed throughout the non-rDNA component of the yeast genome, which comprises 12.5 Mbp of DNA. The physical map suggests that those genes that can be detected and mapped by standard genetic methods are distributed rather uniformly over the full physical extent of the yeast genome. The map has immediate applications to the mapping of genes for which single-copy DNA-DNA hybridization probes are available.
摘要
本文展示了酿酒酵母基因组的物理图谱。它是通过绘制两种限制性内切酶SfiI和NotI的位点而得到的，这两种酶各自识别一个8碱基对的序列。用于遗传定位基因的DNA-DNA杂交探针以及跨越特定SfiI和NotI位点的探针被用来构建一个包含131个物理标记的图谱——32个染色体末端、61个SfiI位点和38个NotI位点。这些标记分布在酵母基因组的非rDNA组分中，该组分包含12.5兆碱基对的DNA。物理图谱表明，那些可以通过标准遗传方法检测和定位的基因在酵母基因组的整个物理范围内分布相当均匀。该图谱可直接应用于那些有单拷贝DNA-DNA杂交探针可用的基因的定位。
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