新一代测序技术概述
Overview of Next-Generation Sequencing Technologies.
作者信息
Slatko Barton E, Gardner Andrew F, Ausubel Frederick M
机构信息
New England Biolabs, Ipswich, Massachusetts.
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts.
出版信息
Curr Protoc Mol Biol. 2018 Apr;122(1):e59. doi: 10.1002/cpmb.59.
Abstract
High throughput DNA sequencing methodology (next generation sequencing; NGS) has rapidly evolved over the past 15 years and new methods are continually being commercialized. As the technology develops, so do increases in the number of corresponding applications for basic and applied science. The purpose of this review is to provide a compendium of NGS methodologies and associated applications. Each brief discussion is followed by web links to the manufacturer and/or web-based visualizations. Keyword searches, such as with Google, may also provide helpful internet links and information. © 2018 by John Wiley & Sons, Inc.
摘要
在过去15年中，高通量DNA测序方法（下一代测序；NGS）迅速发展，新方法不断商业化。随着技术的发展，基础科学和应用科学的相应应用数量也在增加。本综述的目的是提供一份NGS方法及其相关应用的纲要。每次简短讨论之后都附有指向制造商和/或基于网络的可视化工具的网页链接。通过谷歌等进行关键词搜索也可能提供有用的互联网链接和信息。© 2018约翰·威利父子公司版权所有
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