Lorca M, Child R, García A, Silva M, Martínez L, Jerez G, Toledo I, Mezzano D
Departamento de Parasitología, Facultad de Medicina, (Campus Occidente) Universidad de Chile, Santiago de Chile.
Rev Med Chil. 1994 Aug;122(8):925-31.
Aiming to study the applicability and reproducibility of four commercial kits used for the serological detection of Chagas disease (Chagatest-Inst Invest Paraguay, Ortho Chagas, Abbott Chagas (ELISA tests) and Estabilgen Hemo Chagas (indirect hemagglutination test)), a comparative serological study was performed in 256 sera samples coming from a highly endemic area, 249 samples from a low endemic area, 180 reference sera and 2264 samples coming from three blood banks. Specificity of the kits was excellent and sensitivity ranged from 60 to 100%. The indirect hemagglutination test has the lower sensitivity. Some disagreements in the results were observed in the three blood banks, probably due to an unsatisfactory reactive management. We conclude that ELISA tests should be recommended for routine detection of Chagas disease and that for this purpose, a net of laboratories under the direction of a national reference center should exist. This center should assess new commercial products, train technicians and supervise the laboratories.
为研究用于恰加斯病血清学检测的四种商用试剂盒（Chagatest-Inst Invest Paraguay、Ortho Chagas、Abbott Chagas（酶联免疫吸附测定试验）和Estabilgen Hemo Chagas（间接血凝试验））的适用性和可重复性，对来自高流行区的256份血清样本、来自低流行区的249份样本、180份参考血清以及来自三个血库的2264份样本进行了一项比较血清学研究。这些试剂盒的特异性极佳，灵敏度在60%至100%之间。间接血凝试验的灵敏度较低。在三个血库中观察到了一些结果上的差异，这可能是由于反应管理不尽人意所致。我们得出结论，应推荐酶联免疫吸附测定试验用于恰加斯病的常规检测，为此，应设立一个由国家参考中心指导的实验室网络。该中心应评估新的商用产品、培训技术人员并监督各实验室。
