Cabaj W, Stankiewicz M, Jonas W E, Moore L G
Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
Vet Res Commun. 1995;19(1):17-26. doi: 10.1007/BF01839248.
Ten parasite-free lambs were drenched with 8 mg/kg of levamisole on days 0 and 28 and were injected with human erythrocytes and ovalbumin one day after each drench. Ten other antigen-injected lambs were not drenched with anthelmintic as controls. Lymphocytes from the control and drenched lambs were cultured in vitro with RPMI 1640 plus 5% fetal calf serum (FCS), with 50% autologous serum only, with concanavalin A (Con A) or with phytohaemagglutinin (PHA). Decreased blastogenesis was observed in cells from the drenched lambs cultured in the presence or absence of mitogen and was most obvious when 50% autologous serum was used, particularly with PHA, and when lymphocytes were collected 3 and 7 days after the first and 3 days after the second antigen injection. There were no significant changes in antibody titres between the groups. Decreased serum complement activity was seen 3 days after the second antigen injection in the drenched lambs. Although there was a significant reduction in the serum insulin-like growth factor I levels 4 days after each levamisole drench, the drenched lambs gained significantly more weight than the non-drenched control lambs.
10只无寄生虫的羔羊在第0天和第28天以8毫克/千克的剂量灌服左旋咪唑，并在每次灌服后一天注射人红细胞和卵清蛋白。另外10只注射了抗原的羔羊不进行驱虫药灌服作为对照。将对照羔羊和灌服羔羊的淋巴细胞在含有RPMI 1640加5%胎牛血清（FCS）、仅含50%自体血清、伴刀豆球蛋白A（Con A）或植物血凝素（PHA）的培养基中进行体外培养。在有丝分裂原存在或不存在的情况下，灌服羔羊的细胞均出现增殖降低，当使用50%自体血清时最为明显，尤其是使用PHA时，以及在首次抗原注射后3天和7天以及第二次抗原注射后3天收集淋巴细胞时。两组之间抗体滴度无显著变化。灌服羔羊在第二次抗原注射后3天血清补体活性降低。尽管每次灌服左旋咪唑后4天血清胰岛素样生长因子I水平显著降低，但灌服羔羊的体重显著高于未灌服的对照羔羊。
