Kaneene J M, Okino F C, Anderson R K, Muscoplat C C, Johnson D W
Vet Immunol Immunopathol. 1981 Feb;2(1):75-85. doi: 10.1016/0165-2427(81)90040-4.
Incubation of Brucella abortus (field strain) infected and strain 19 vaccinated bovine peripheral blood lymphocytes with B. abortus antigen and levamisole caused a consistently significant increase in [3H] thymidine uptake when compared to cultures without levamisole. Levamisole did not potentiate B. abortus-induced blastogenic response of lymphocytes from non-exposed cattle. A dose response study showed that 10 micrograms/culture induced maximum potentiation of B. abortus-induced lymphocyte stimulation. Using the 10 micrograms/well concentration of levamisole, further studies were conducted to determine the net potentiation of the blastogenic responses in lymphocytes from B. abortus (field strain) infected cattle. B. abortus strain 19 vaccinated but nonresponsive and non-exposed cattle. Levamisole significantly potentiated the B. abortus-induced lymphocyte blastogenesis in lymphocytes from unresponsive cattle.
将感染布鲁氏菌流产株（野毒株）和接种19号菌苗的牛外周血淋巴细胞与布鲁氏菌流产株抗原及左旋咪唑一起孵育，与未添加左旋咪唑的培养物相比，[3H]胸腺嘧啶核苷摄取量持续显著增加。左旋咪唑不能增强未接触过布鲁氏菌的牛淋巴细胞对布鲁氏菌流产株诱导的增殖反应。剂量反应研究表明，每培养物10微克可诱导布鲁氏菌流产株诱导的淋巴细胞刺激的最大增强作用。使用每孔10微克的左旋咪唑浓度，进一步研究以确定布鲁氏菌流产株（野毒株）感染牛、接种19号菌苗但无反应的牛以及未接触过布鲁氏菌的牛淋巴细胞增殖反应的净增强作用。左旋咪唑显著增强了无反应牛淋巴细胞对布鲁氏菌流产株诱导的淋巴细胞增殖作用。
