Rojas J M, Dopazo J, Santana M, López-Galíndez C, Tabarés E
Centro Nacional de Biología Celular y Retrovirus, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
Virus Res. 1995 Feb;35(2):205-14. doi: 10.1016/0168-1702(94)00097-v.
The variability of herpes simplex viruses has been measured using the RNAse A mismatch cleavage method in two genes: thymidine kinase and glycoprotein B of both HSV-1 and HSV-2. This technique permitted us to study the variability of the virus with a greater level of resolution than restriction endonuclease analysis. The phylogenetic trees obtained for the different genes allowed us to identify consistent clusters of viruses circulating in the same geographical area. Our results showed that thymidine kinase is more heterogeneous than glycoprotein B for both subtypes of HSV, and confirmed that HSV-1 is more heterogeneous than HSV-2 for both genes. This is the first time that this kind of analysis has been applied to DNA viruses.
已使用核糖核酸酶A错配切割法在两个基因中测量单纯疱疹病毒的变异性，这两个基因分别是1型和2型单纯疱疹病毒的胸苷激酶和糖蛋白B。与限制性内切酶分析相比，该技术使我们能够以更高的分辨率研究病毒的变异性。从不同基因获得的系统发育树使我们能够识别在同一地理区域传播的病毒的一致聚类。我们的结果表明，对于单纯疱疹病毒的两种亚型，胸苷激酶比糖蛋白B更具异质性，并证实对于这两个基因，1型单纯疱疹病毒比2型单纯疱疹病毒更具异质性。这是此类分析首次应用于DNA病毒。
