Olin K L, Golub M S, Gershwin M E, Hendrickx A G, Lonnerdal B, Keen C L
Department of Nutrition, University of California at Davis 95616-8669, USA.
Am J Clin Nutr. 1995 Jun;61(6):1263-7. doi: 10.1093/ajcn/61.6.1263.
Assessment of zinc nutriture is often compromised by the lack of reliable biomarkers. In the present study the effect of dietary zinc deprivation on plasma extracellular superoxide dismutase (EC SOD) activity was investigated in rat and rhesus macaque models. This enzyme, which contains both zinc and copper, is distinct from cytosolic copper-zinc SOD. Young, growing rats fed zinc-deficient diets (1.5 nmol Zn/g diet) were characterized by low plasma zinc concentrations and plasma EC SOD activities (16% and 56% of controls, respectively). Adolescent rhesus macaques fed diets that contained a marginal amount of zinc (30.6nmol Zn/g diet) also had low plasma zinc concentrations and low EC SOD activities compared with controls fed diets containing 765 nmol Zn/g diet (75% and 40%, respectively). Enzyme activity was not affected after in vitro addition of zinc to plasma samples from control, restrict-fed, and zinc-deficient rats. Taken together, these data support the concept that plasma EC SOD activity can be a biomarker for zinc status.
锌营养状况的评估常常因缺乏可靠的生物标志物而受到影响。在本研究中，在大鼠和恒河猴模型中研究了膳食锌缺乏对血浆细胞外超氧化物歧化酶（EC SOD）活性的影响。这种同时含有锌和铜的酶与胞质铜锌超氧化物歧化酶不同。喂食缺锌日粮（1.5 nmol锌/克日粮）的幼年生长大鼠的特征是血浆锌浓度和血浆EC SOD活性较低（分别为对照组的16%和56%）。与喂食含765 nmol锌/克日粮的对照组相比，喂食含微量锌日粮（30.6 nmol锌/克日粮）的青春期恒河猴的血浆锌浓度和EC SOD活性也较低（分别为75%和40%）。对来自对照、限食和缺锌大鼠的血浆样本进行体外补锌后，酶活性未受影响。综上所述，这些数据支持血浆EC SOD活性可作为锌状态生物标志物的概念。
