类风湿性关节炎和骨关节炎患者培养滑膜细胞中白细胞介素-1受体拮抗剂的产生
Interleukin-1 receptor antagonist production in cultured synovial cells from patients with rheumatoid arthritis and osteoarthritis.
作者信息
Fujikawa Y, Shingu M, Torisu T, Masumi S
机构信息
Department of Orthopaedic Surgery, Oita Medical University, Japan.
出版信息
Ann Rheum Dis. 1995 Apr;54(4):318-20. doi: 10.1136/ard.54.4.318.
OBJECTIVE
To measure the amounts of interleukin-1 receptor antagonist (IL-1ra) protein produced by cultured synovial cells obtained from patients with rheumatoid arthritis (RA) and osteoarthritis (OA).
METHODS
Synovial cells obtained from patients with either RA or OA were cultured and the supernatants were measured for IL-1ra by enzyme linked immunosorbent assay.
RESULTS
The synovial cells obtained from patients with RA produced significantly smaller amounts of IL-1ra than did those obtained from patients with OA, in a late passage (third to fifth) without stimulation and a first passage both with and without stimulation (p < 0.025, respectively). In addition, when the patients with RA were divided into two groups according to the maximum number of lining cell layers, the amounts of IL-1ra produced by the proliferative type were smaller than those produced by the less proliferative type (p < 0.025).
CONCLUSIONS
The above findings suggest that IL-1ra production in RA synovial cells is suppressed, and that reduced IL-1ra protein production is one of the causes which leads to the proliferation of lining cells and persistent joint inflammation.
目的
测量从类风湿关节炎（RA）和骨关节炎（OA）患者获取的培养滑膜细胞产生的白细胞介素-1受体拮抗剂（IL-1ra）蛋白量。
方法
培养从RA或OA患者获取的滑膜细胞，通过酶联免疫吸附测定法测量上清液中的IL-1ra。
结果
在未刺激的晚期传代（第三代至第五代）以及有刺激和无刺激的第一代传代中，从RA患者获取的滑膜细胞产生的IL-1ra量显著少于从OA患者获取的滑膜细胞（p分别<0.025）。此外，当根据衬里细胞层的最大数量将RA患者分为两组时，增殖型产生的IL-1ra量少于增殖较少型（p<0.025）。
结论
上述发现表明，RA滑膜细胞中IL-1ra的产生受到抑制，IL-1ra蛋白产生减少是导致衬里细胞增殖和持续性关节炎症的原因之一。
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