Chomarat P, Vannier E, Dechanet J, Rissoan M C, Banchereau J, Dinarello C A, Miossec P
Laboratory for Immunologic Research, Schering-Plough, Dardilly, France.
J Immunol. 1995 Feb 1;154(3):1432-9.
The spontaneous production of IL-1 beta (IL-1 beta) and IL-1 receptor antagonist (IL-1Ra) by rheumatoid arthritis (RA) synovium, and the regulation of their production by IL-4 and IL-10, were studied. Supernatants from cultured synovium pieces from 19 RA patients were assayed for IL-1 beta and IL-1Ra production using ELISA and RIA, respectively. After 10 days of culture, spontaneous production of IL-1Ra was 1.42 +/- 0.43 ng/ml/100 mg of synovium whereas spontaneous production of IL-1 beta was 4.03 +/- 0.90 ng/ml/100 mg of synovium (n = 19). The addition of IL-4 reduced IL-1 beta production by 2.3-fold (p = 0.001) and increased that of IL-1Ra by 2.8-fold (p = 0.003). IL-10 had no significant effect on IL-1Ra production and suppressed IL-1 beta production (primarily in samples producing high levels of IL-1 beta). However, IL-10 was less potent than IL-4 in suppressing IL-1 beta production. IL-1Ra was mainly produced by rheumatoid synovial monocytes/macrophages. IL-4 was more potent than IL-10 in inducing IL-1Ra production by monocytes/macrophages purified from RA synovium, as well as from RA blood. Thus, RA synovium is characterized by an imbalance between IL-1Ra and IL-1 beta production, in favor of the latter. IL-4, and to a lesser extent IL-10, shift this balance in favor of an anti-inflammatory situation.
研究了类风湿关节炎（RA）滑膜中白细胞介素-1β（IL-1β）和白细胞介素-1受体拮抗剂（IL-1Ra）的自发产生，以及IL-4和IL-10对其产生的调节作用。分别采用酶联免疫吸附测定法（ELISA）和放射免疫测定法（RIA）检测了19例RA患者滑膜组织培养块上清液中IL-1β和IL-1Ra的产生情况。培养10天后，IL-1Ra的自发产生量为1.42±0.43 ng/ml/100 mg滑膜，而IL-1β的自发产生量为4.03±0.90 ng/ml/100 mg滑膜（n = 19）。添加IL-4可使IL-1β的产生减少2.3倍（p = 0.001），使IL-1Ra的产生增加2.8倍（p = 0.003）。IL-10对IL-1Ra的产生无显著影响，但可抑制IL-1β的产生（主要在产生高水平IL-1β的样本中）。然而，IL-10在抑制IL-1β产生方面的作用不如IL-4强。IL-1Ra主要由类风湿滑膜单核细胞/巨噬细胞产生。在诱导从RA滑膜以及RA血液中纯化的单核细胞/巨噬细胞产生IL-1Ra方面，IL-4比IL-10更有效。因此，RA滑膜的特征是IL-1Ra和IL-1β产生之间失衡，有利于后者。IL-4以及程度较轻的IL-10可使这种平衡向有利于抗炎的方向转变。
