Takeshita K, Ishibashi H, Suzuki M, Yamamoto T, Akaike T, Kodama M
First Department of Surgery, Shiga University of Medical Science, Japan.
Artif Organs. 1995 Feb;19(2):191-3. doi: 10.1111/j.1525-1594.1995.tb02310.x.
A compact three-dimensional (3D) module is needed for hepatocyte culture in order to develop an effective hybrid artificial liver system that can retain hepatocellular structure and differentiated functions. We treated the 3D module with collagen gel to entrap rat hepatocytes. This method yielded a high hepatocellular density (2 x 10(7) cells/ml) over a period of 14 days and maintained the secretion of albumin and ureogenesis at the same level as the control monolayer method. The ammonia removal remained at 43% of the Day 0 value over 8 days of perfusion. Our data show that this approach may be useful for liver support therapy in an extracorporeal circuit.
为了开发一种能够保留肝细胞结构和分化功能的有效混合人工肝系统，肝细胞培养需要一个紧凑的三维（3D）模块。我们用胶原凝胶处理3D模块以包埋大鼠肝细胞。该方法在14天内产生了高肝细胞密度（2×10⁷个细胞/毫升），并将白蛋白分泌和尿素生成维持在与对照单层方法相同的水平。在8天的灌注过程中，氨去除率保持在第0天值的43%。我们的数据表明，这种方法可能对体外循环中的肝支持治疗有用。
