Suzuki M, Takeshita K, Yamamoto T, Ishibashi H, Kodama M
First Department of Surgery, Shiga University of Medical Science, Japan.
Artif Organs. 1997 Feb;21(2):99-106. doi: 10.1111/j.1525-1594.1997.tb00345.x.
Preservation of hepatocytes is a key technical factor toward the successful clinical application of hybrid artificial livers. It was possible to culture hepatocytes that had been preserved with collagen gel for 8 and 14 days in 4 degrees C University of Wisconsin solution. Phase-difference and scanning electron microscopy showed that most of the stored hepatocytes maintained a round-shaped morphology. In the 14 day preservation group, on Days 2 and 8, respectively, ureogenesis was 98.3% and 69.6%, gluconeogenesis was 65.2% and 80.7%, lidocaine clearance was 81.7% and 72.5%, urea synthesis after ammonia load was 47.6% and 57.5% of those in the comparable control group. This implies that preserved hepatocytes maintained adequate functional capability even after 14 days of preservation. We suggest that our preservation method will be valuable for the future application and development of a practical hybrid artificial liver.
肝细胞的保存是混合人工肝成功临床应用的关键技术因素。在4℃的威斯康星大学溶液中，用胶原凝胶保存8天和14天的肝细胞仍可进行培养。相差显微镜和扫描电子显微镜显示，大多数储存的肝细胞保持圆形形态。在14天保存组中，分别在第2天和第8天，尿素生成量分别为对照组的98.3%和69.6%，糖异生分别为65.2%和80.7%，利多卡因清除率分别为81.7%和72.5%，氨负荷后尿素合成量分别为对照组的47.6%和57.5%。这意味着即使保存14天后，保存的肝细胞仍保持足够的功能能力。我们认为我们的保存方法对未来实用型混合人工肝的应用和发展将具有重要价值。
