Brodelius P, Pedersen H
Department of Plant Biochemistry, University of Lund, Sweden.
Trends Biotechnol. 1993 Jan;11(1):30-6. doi: 10.1016/0167-7799(93)90072-H.
Various approaches for redirecting transport of secondary metabolites in plant-cell suspension cultures have been attempted in an effort to increase productivity. Little is understood of the transport mechanisms or their regulation, and many of the extractive methods which involve membrane permeabilization have an adverse effect on cell viability. Increasing the activity of metabolic pathways by elicitation, in conjunction with end-product removal and accumulation in an extractive phase, has proven to be the most successful strategy so far.
为了提高产量，人们尝试了各种方法来改变植物细胞悬浮培养物中次生代谢产物的转运。目前对转运机制及其调控了解甚少，而且许多涉及膜通透化的提取方法对细胞活力有不利影响。事实证明，通过诱导提高代谢途径的活性，同时结合终产物在提取阶段的去除和积累，是迄今为止最成功的策略。
