Kutchan T M
Lehrstuhl für Pharmazeutische Biologie, Universität München, Germany.
Phytochemistry. 1993 Feb;32(3):493-506. doi: 10.1016/s0031-9422(00)95128-8.
In this review, the elucidation of the structure of the first key alkaloidal intermediate in monoterpenoid indole alkaloid biosynthesis, 3 alpha(S)-strictosidine, is presented. The discovery of the enzyme which catalyses the stereospecific formation of this alkaloidal precursor from tryptamine and secologanin, strictosidine synthase, is also detailed. From the knowledge provided by the stereochemical structure of strictosidine and the biochemical characteristics of the biosynthetic enzyme, strictosidine synthase, a new approach to the study of monoterpenoid indole alkaloid biosynthesis was developed. Physiological studies of monoterpenoid indole alkaloid biosynthesis at the enzymic level in plants and plant cell cultures were performed followed by the analyses of these systems at the level of molecular genetics.
在本综述中，介绍了单萜吲哚生物碱生物合成中第一个关键生物碱中间体3α(S)-士的宁苷的结构解析。还详细阐述了催化从色胺和裂环马钱子苷立体特异性形成这种生物碱前体的酶——士的宁苷合酶的发现。基于士的宁苷的立体化学结构和生物合成酶士的宁苷合酶的生化特性所提供的知识，开发了一种研究单萜吲哚生物碱生物合成的新方法。对植物和植物细胞培养物中酶水平的单萜吲哚生物碱生物合成进行了生理学研究，随后在分子遗传学水平上对这些系统进行了分析。
