Ganeva V J, Tsoneva I C
Department of Biophysics and Radiobiology, Sofia, Bulgaria.
Appl Microbiol Biotechnol. 1993 Mar;38(6):795-8. doi: 10.1007/BF00167147.
The correlation between electrotransformation and electropermeability was studied in yeast cells following the modification of their membranes by treatment with n-alcohols. It was found that the number of transformed cells decreased with increase of chain length of the alcohols used as follows: methanol < ethanol < propanol < butanol. The electropermeability was unaffected by the prepulse n-alcohol treatment. The lack of a unidirectional link between permeability and transformation leads to the assumption that the mechanism of DNA introduction into the cell could not be interpreted solely as a result of the existence of pores.
在用正醇处理对酵母细胞膜进行修饰后，研究了电转化与电通透性之间的相关性。结果发现，随着所用醇类链长的增加，转化细胞的数量减少，顺序如下：甲醇＜乙醇＜丙醇＜丁醇。预脉冲正醇处理对电通透性没有影响。通透性与转化之间缺乏单向联系，这导致人们认为，DNA导入细胞的机制不能仅仅归因于孔的存在。
