Marks J D, Ouwehand W H, Bye J M, Finnern R, Gorick B D, Voak D, Thorpe S J, Hughes-Jones N C, Winter G
MRC Centre for Protein Engineering, Cambridge, UK.
Biotechnology (N Y). 1993 Oct;11(10):1145-9. doi: 10.1038/nbt1093-1145.
We have isolated human antibody fragments with binding specificities against the blood group antigens of the ABO and I blood group systems (B and HI), of the Rh system (D and E) and of the Kell system (Kpb), by selecting a phage-antibody library on human red cells. The fragments, expressed in bacteria, were antigen-specific and effective in assays including agglutination and immunohistochemistry. Selection of phage-antibody libraries using intact cells seems to offer a promising alternative to hybridoma technology for the production of antibodies against cell surface molecules.
我们通过在人红细胞上筛选噬菌体抗体文库，分离出了具有针对ABO和I血型系统（B和H抗原）、Rh系统（D和E抗原）以及Kell系统（Kpb抗原）血型抗原结合特异性的人抗体片段。这些在细菌中表达的片段具有抗原特异性，并且在包括凝集和免疫组织化学在内的检测中有效。使用完整细胞筛选噬菌体抗体文库似乎为生产针对细胞表面分子的抗体提供了一种比杂交瘤技术更有前景的替代方法。
