Courtney-Gutterson N, Napoli C, Lemieux C, Morgan A, Firoozabady E, Robinson K E
DNA Plant Technology Corporation, Oakland, CA 94608.
Biotechnology (N Y). 1994 Mar;12(3):268-71. doi: 10.1038/nbt0394-268.
Chimeric chalcone synthase (CHS) constructs were prepared in both anti-sense and sense orientations, and introduced into the chrysanthemum cultivar Moneymaker, along with a T-DNA vector lacking a CHS construct. For both the anti-sense and sense constructs, the majority of the plants produced pink flowers typical of Moneymaker itself. Of 133 sense and 83 anti-sense transgenic individuals 3 of each set produced fully white or very pale pink flowers. No white-flowering transgenic plants were obtained in control transformations. The white flowers were found to accumulate higher levels of chalcone synthase precursors and to have reduced levels of chalcone synthase message. A small-scale field trial was performed to evaluate the stability of the phenotype throughout a series of vegetative propagation steps and during plant growth. The white-flowering trait was maintained well through vegetative propagation; however, during growth of individual white-flowering plants, some pink color was found in some flowers. At one site 2% of the white-flowering plants produced a few pink flowers; at two other sites, as many as 10-12% of the plants produced pale pink flowers.
构建了反义方向和正义方向的嵌合查尔酮合酶（CHS）构建体，并将其与缺乏CHS构建体的T-DNA载体一起导入菊花品种“金钱菊”。对于反义构建体和正义构建体，大多数植株都开出了“金钱菊”本身特有的粉色花朵。在133株正义转基因个体和83株反义转基因个体中，每组各有3株开出了完全白色或非常浅粉色的花朵。在对照转化中未获得开白花的转基因植株。发现白花积累了更高水平的查尔酮合酶前体，且查尔酮合酶信使水平降低。进行了一项小规模田间试验，以评估该表型在一系列营养繁殖步骤以及植物生长过程中的稳定性。白花性状通过营养繁殖得到了很好的保持；然而，在个别开白花的植株生长过程中，一些花朵中出现了一些粉色。在一个地点，2%的开白花植株开出了几朵粉色花朵；在另外两个地点，多达10%-12%的植株开出了浅粉色花朵。
