Koizumi K, Tanimoto T, Kubota Y, Kitahata S
School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Carbohydr Res. 1997 Dec;305(3-4):393-400. doi: 10.1016/s0008-6215(97)00181-x.
Novel branched cycloisomalto-octaoses (CI8s) were enzymatically synthesized by transgalactosylation with alpha-galactosidase from coffee bean and beta-galactosidase preparations from Penicillium multicolor and Bacillus circulans, using melibiose and lactose as donor substrates, and CI8 which is a cyclic homogeneous oligosaccharide composed of eight glucose units bound by alpha-(1-->6)-linkages, as an acceptor. alpha-Galactosyl-CI8s and beta-galactosyl-CI8s obtained were isolated and purified by HPLC. Their structures were elucidated by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDITOFMS) and NMR spectroscopy.
利用咖啡豆中的α-半乳糖苷酶以及多色青霉和环状芽孢杆菌中的β-半乳糖苷酶，通过转半乳糖基化反应，以蜜二糖和乳糖作为供体底物，以由八个通过α-(1→6)-键连接的葡萄糖单元组成的环状均一寡糖CI8作为受体，酶法合成了新型支链环麦芽八糖（CI8s）。通过高效液相色谱法（HPLC）分离并纯化得到了α-半乳糖基-CI8s和β-半乳糖基-CI8s。通过基质辅助激光解吸电离飞行时间质谱（MALDITOFMS）和核磁共振光谱（NMR）对它们的结构进行了阐明。
