Merkler D J
Analytical Protein and Organic Chemistry Group, Unigene Laboratories, Inc., Fairfield, NJ 07004.
Enzyme Microb Technol. 1994 Jun;16(6):450-6. doi: 10.1016/0141-0229(94)90014-0.
Peptidylglycine alpha-amidating enzyme (alpha-AE) can be used in an in vitro reaction to convert C-terminal glycine-extended peptides to peptide hormones with a C-terminal amino acid amide. Structure-activity data for 45 bioactive peptides show that the C-terminal amide is required for the full biological activity of most amidated peptide hormones. These data emphasize the role alpha-AE can have in amidated peptide production.
肽基甘氨酸α-酰胺化酶（α-AE）可用于体外反应，将C末端带有甘氨酸延伸的肽转化为具有C末端氨基酸酰胺的肽激素。45种生物活性肽的构效关系数据表明，C末端酰胺对于大多数酰胺化肽激素的完全生物活性是必需的。这些数据强调了α-AE在酰胺化肽生产中可能发挥的作用。
