Life Technologies, Inc., Grand Island, NY, USA.
Cytotechnology. 1997 Jan;23(1-3):95-101. doi: 10.1023/A:1007967602484.
The evolution of basal synthetic formulations to support mammalian cell culture applications has been facilitated by the contributions of many investigators. Definition of minimally-required nutrient categories by Harry Eagle in the 1950's spawned an iterative process of continuous modification and refinement of the exogenous environment to cultivate new cell types and to support emerging applications of cultured mammalian cells. Key historical elements are traced, leading to the development of high potency, basal nutrient formulations capable of sustaining serum-free proliferation and biological production. Emerging techniques for alimentation of fed batch and continuous perfusion bioreactors, using partial nutrient concentrates deduced from spent medium analysis, can enhance medium utilization and bioreactor productivity.
基础合成配方的发展演变，促进了哺乳动物细胞培养应用的发展。20 世纪 50 年代，哈里·伊格尔（Harry Eagle）定义了最基本的营养类别，由此开始了一个不断修改和完善外环境的迭代过程，以培养新的细胞类型，并支持新兴的哺乳动物细胞培养应用。本文追溯了关键的历史元素，最终开发出了高效能的基础营养配方，能够支持无血清增殖和生物生产。从消耗培养基分析中推导出来的部分营养浓缩物，用于补料分批和连续灌注生物反应器的喂养技术，能够提高培养基的利用率和生物反应器的生产力。
