Liljeström P
Center for Biotechnology, Karolinska Institute, Huddinge, Sweden.
Curr Opin Biotechnol. 1994 Oct;5(5):495-500. doi: 10.1016/0958-1669(94)90063-9.
Alphavirus vectors are newcomers in the field of heterologous gene expression. Nevertheless, they have rapidly become popular and are now being used in a wide range of applications. During the past year, new vectors and new methods for their use have improved levels of gene expression. As alphaviruses are capable of infecting humans, biosafety was an important issue during early work with these vectors. The construction of a conditional lethal helper system has now largely overcome this problem, and should further increase the utility of these types of vector in animal cell systems.
甲病毒载体是异源基因表达领域的新成员。然而，它们迅速受到欢迎，目前正被广泛应用于各种领域。在过去的一年里，新的载体及其使用的新方法提高了基因表达水平。由于甲病毒能够感染人类，在早期使用这些载体的工作中，生物安全性是一个重要问题。条件致死辅助系统的构建现已在很大程度上克服了这一问题，并且应该会进一步提高这类载体在动物细胞系统中的实用性。
