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Pulsed-field gel electrophoretic identification of Salmonella enterica serovar Typhimurium live vaccine strain Zoosaloral H.

作者信息

Schwarz S, Liebisch B

机构信息

Institut für Kleintierforschung Celle/Merbitz der Bundesforschungsanstalt für Landwirtschaft, Celle, Germany.

出版信息

Lett Appl Microbiol. 1994 Dec;19(6):469-72. doi: 10.1111/j.1472-765x.1994.tb00984.x.

DOI:10.1111/j.1472-765x.1994.tb00984.x
PMID:7765707
Abstract

Salmonella enterica subsp. enterica serovar Typhimurium (Salm. Typhimurium) live vaccine strain Zoosaloral H was characterized by pulsed-field gel electrophoresis (PFGE). Each of the two suitable restriction enzymes, XbaI and SpeI, produced a unique restriction fragment pattern for this live vaccine strain which was not shared by field isolates of the same serovar. The characteristic fragment pattern proved to be stable during a 22 month observation period and was also not altered after animal passage of the vaccine strains. Thus PFGE analysis proved to be a helpful tool in the identification of Salm. Typhimurium live vaccine strain Zoosaloral H and its differentiation from wild-type isolates of the same serovar.

摘要

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