鉴定牛分枝杆菌卡介苗的一种25千道尔顿蛋白以区分卡介苗菌株与结核分枝杆菌。
Identification of a 25-kilodalton protein of Mycobacterium bovis BCG to distinguish BCG strains from Mycobacterium tuberculosis.
作者信息
Kumar D, Srivastava B S, Singh N B, Srivastava R
机构信息
Division of Microbial Genetics, Central Drug Research Institute, Lucknow, India.
出版信息
J Clin Microbiol. 1996 Jan;34(1):224-6. doi: 10.1128/jcm.34.1.224-226.1996.
Abstract
Mycobacterium bovis BCG vaccine strains were compared with Mycobacterium tuberculosis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A 25-kDa protein observed in the BCG strains was absent in M. tuberculosis. Rabbit antibodies specific to the 25-kDa protein uniquely identified this protein in BCG strains but not in M. tuberculosis. It is suggested that the 25-kDa protein and polyclonal antibodies directed against this antigen can be exploited to distinguish BCG strains from M. tuberculosis.
摘要
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，将牛分枝杆菌卡介苗疫苗株与结核分枝杆菌进行了比较。在卡介苗菌株中观察到的一种25 kDa蛋白在结核分枝杆菌中不存在。针对该25 kDa蛋白的兔抗体能特异性识别卡介苗菌株中的这种蛋白，而不能识别结核分枝杆菌中的该蛋白。有人提出，这种25 kDa蛋白和针对该抗原的多克隆抗体可用于区分卡介苗菌株和结核分枝杆菌。
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