Yu Z, Mohn W W
Department of Microbiology and Immunology, and Pulp and Paper Centre, University of British Columbia, Vancouver, Canada.
Water Res. 2001 Mar;35(4):883-90. doi: 10.1016/s0043-1354(00)00335-3.
Resin acids are the major toxicants in pulp and paper mill effluents (PPMEs), and they form pitch interfering with papermaking. Efficient and reliable resin acid removal is critically important to prevent toxicity discharge and ensure proper functioning of paper machines. Two resin-acid-degrading bacteria, Pseudomonas abietaniphila BKME-9 and Zoogloea resiniphila DhA-35, were tested in laboratory sequencing batch reactors (SBRs) for their ability to enhance resin acid removal by biomass from a full-scale biotreatment system treating PPMEs. Both bacteria enhanced resin acid removal but not removal of total organic carbon (TOC) by either pH-shocked or starved activated sludge. These two bacteria also increased resin acid removal when the sludge was given high concentration (200 microM) of resin acid. A most-probable-number polymerase chain reaction (MPN-PCR) assay showed that these two bacteria were initially not detectable (detection limit: 10(2) bacterial cells/ml) in the sludge community and were persistent after inoculation. Both bacteria did not substantially change the indigenous microbial community composition, as assayed by ribosomal intergenic spacer analysis (RISA). Our results suggest that it is feasible and potentially useful to enhance resin acid removal by bioaugmentation using resin-acid-degrading bacteria such as BKME-9 and DhA-35.
树脂酸是制浆造纸厂废水（PPMEs）中的主要有毒物质，它们会形成沥青，干扰造纸过程。高效可靠地去除树脂酸对于防止毒性排放和确保造纸机正常运行至关重要。在实验室序批式反应器（SBRs）中测试了两种树脂酸降解细菌，嗜冷假单胞菌BKME-9和树脂嗜动胶菌DhA-35，它们通过生物量增强从处理PPMEs的全规模生物处理系统中去除树脂酸的能力。这两种细菌都增强了树脂酸的去除，但无论是pH冲击还是饥饿的活性污泥都没有增强总有机碳（TOC）的去除。当污泥中加入高浓度（200 microM）的树脂酸时，这两种细菌也增加了树脂酸的去除。一种最可能数聚合酶链反应（MPN-PCR）检测表明，这两种细菌最初在污泥群落中无法检测到（检测限：10(2)个细菌细胞/ml），接种后仍能持续存在。通过核糖体基因间隔区分析（RISA）测定，这两种细菌都没有显著改变本地微生物群落组成。我们的结果表明，使用BKME-9和DhA-35等树脂酸降解细菌通过生物强化来增强树脂酸去除是可行的，并且可能是有用的。
