Chagnaud J L, Amara A, Faiderbe S, Geffard M
Laboratoire d'Immunologie et Pathologie, Université de Bordeaux II, France.
Cancer Lett. 1995 May 8;91(2):253-60. doi: 10.1016/0304-3835(95)03738-i.
Anti-benzo[a]pyrene (B[a]P)-like autoantibodies (autoAb) have been characterized in sera of patients with epithelial tumors. Circulating immune complexes (CIC) from these sera have been analysed after polyacrylamide gel electrophoresis (PAGE) under non-denaturing conditions. Immunoblotting was performed using a monoclonal antiidiotypic antibody (Ab), internal image of conjugated B[a]P called AIB1 and anti-human immunoglobulins (Ig). An immunoreactivity was seen only with AIB1 Ab, suggesting the presence of a 'B[a]P-like' binding protein. Additional studies showed that this immunoreactivity is not associated with an 18- to 20-kDa protein previously identified in the same CIC.
在上皮肿瘤患者血清中已鉴定出抗苯并[a]芘（B[a]P）样自身抗体（自身抗体）。在非变性条件下进行聚丙烯酰胺凝胶电泳（PAGE）后，对这些血清中的循环免疫复合物（CIC）进行了分析。使用单克隆抗独特型抗体（抗体）、称为AIB1的结合B[a]P的内影像以及抗人免疫球蛋白（Ig）进行免疫印迹。仅用AIB1抗体观察到免疫反应性，表明存在一种“B[a]P样”结合蛋白。进一步研究表明，这种免疫反应性与先前在同一CIC中鉴定出的18至20 kDa蛋白无关。
